Public Health and Environmental Laboratories

Harvest Order Choice: 60060

Synonyms:  Salmonella Culture; Shigella Culture; Escherichia coli O157 Culture; STEC Culture; Yersinia Culture; Vibrio Culture; Listeria monocytogenes Culture

Program: Microbiology

Unit: Enterics

Useful For: Testing food sample(s) from epidemiology or local health departments for foodborne disease such as Salmonella, Shigella, E. coli, Yersinia, Vibrio and Listeria monocytogenes.

Method: Vidas, Culture &Vitek II

Charge: N/A

Request Form: LAB-3 (Chain of custody)

PHEL Order Choice #: 60040

Synonyms:

Submission Form: BACT-109

Program: Microbiology

Unit: Enterics

Use: Surveillance

Method: Culture and Identification

Platform: Manual, VITEK, ELISA, PCR, Whole Genome Sequencing

Organism: Listeria monocytogenes, Salmonella spp., Shigella spp., Escherichia coli., Yersinia enterocolitica, Vibrio spp.

Preferred Specimen Type: Isolate

Accepted Specimen Type: Isolate, stool (for shiga-toxin producing E. coli testing only)

Specimen Volume Requirements: Stool samples:1-5 grams

Submission Requirements: Submit fresh (24-48 hour) cultures on SBA, TSA or Chocolate agar slants, for shiga-toxin producing E. coli: Submit fresh 24-hour STEC broths.

Storage Temperature: Room temperature (15-30C) or refrigerated (2-8C) (Vibrio refrigerated only)

Transport Requirements: 15-30C